The Monoamine Oxidase Assay Kit provides a convenient fluorimetric
means to measure MAO enzyme activity in biological samples. In the assay, MAO
reacts with p-tyramine, a substrate for both MAO-A and MAO-B, resulting in the
formation of H2O2, which is determined by a fluorimetric method. The assay is
simple, sensitive, stable, and high-throughput adaptable. Monoamine Oxidase
Assay Kit provides a sensitive and simple fluorometric method for detecting
monoamine oxidase activity in purified enzyme preparations at levels as low as
12 µU/mL and in tissue samples with as little as 20 µg protein in a 100 µL
assay volume.
Monoamine Oxidase Assay Kit
applies the competitive enzyme immunoassay technique utilizing a
monoclonal anti-MAO antibody and an MAO-HRP conjugate. The MAO Assay kit
provides a homogeneous luminescent method for measuring monoamine oxidase (MAO)
activity from recombinant and native sources and for testing the effects of
test compounds on MAO activity. The MAO Assay kit is performed by incubating
the MAO enzyme source with a luminogenic MAO substrate. The substrate of the
MAO Assay is a derivative of beetle luciferin. Upon reaction with MAO, the
derivative is converted into luciferin, which in turn reacts with luciferase to
produce light. The amount of light produced is directly proportional to the
activity of MAO.