Monoamine Oxidase Kit

The Monoamine Oxidase Assay Kit provides a convenient fluorimetric means to measure MAO enzyme activity in biological samples. In the assay, MAO reacts with p-tyramine, a substrate for both MAO-A and MAO-B, resulting in the formation of H2O2, which is determined by a fluorimetric method. The assay is simple, sensitive, stable, and high-throughput adaptable. Monoamine Oxidase Assay Kit provides a sensitive and simple fluorometric method for detecting monoamine oxidase activity in purified enzyme preparations at levels as low as 12 µU/mL and in tissue samples with as little as 20 µg protein in a 100 µL assay volume.

Monoamine Oxidase Assay Kit  applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MAO antibody and an MAO-HRP conjugate. The MAO Assay kit provides a homogeneous luminescent method for measuring monoamine oxidase (MAO) activity from recombinant and native sources and for testing the effects of test compounds on MAO activity. The MAO Assay kit is performed by incubating the MAO enzyme source with a luminogenic MAO substrate. The substrate of the MAO Assay is a derivative of beetle luciferin. Upon reaction with MAO, the derivative is converted into luciferin, which in turn reacts with luciferase to produce light. The amount of light produced is directly proportional to the activity of MAO.